Plasmonic and structural properties of silver nanoparticles fabricated by the metallisation of DNA
Type
conference paper
Date Issued
2007-05-10
Author(s)
Abstract
The growth of metallic nanoparticles along synthetic DNA templates constitutes a highly promising new route to yield nanoparticles of well controlled size and shape. Further, taking advantage of the superior recognition characteristics of nucleic acids, it should be possible to arrange DNA-based nanoparticles in self-assembled structures with defined inter-particle spacing.
In this study, we use two different methods to deposit silver on oligonucleotides of different lengths (23-96 base pairs) in order to synthesize metallic nanorods with controlled aspect ratios. The first method involves the specific labeling of nucleotides with aldehyde groups, followed by the exposure to Tollens reagent and a development solution, whereas the second method relies on the photoinduced deposition of Ag onto unmodified DNA samples. Several preparation parameters (DNA sequence, buffer salt type, Ag concentration, UV illumination time) are varied systematically.
The obtained DNA-templated nanoparticles are characterized with respect to their optical and structural properties. Plasmonic modes can be identified from maxima observed in the extinction spectra. The size and shape of the nanoparticles is inferred from conducting polarized dynamic-light-scattering experiments in the solution phase, as well as from imaging dispersed nanoparticles on singlecrystalline substrates by atomic force microscopy.
In this study, we use two different methods to deposit silver on oligonucleotides of different lengths (23-96 base pairs) in order to synthesize metallic nanorods with controlled aspect ratios. The first method involves the specific labeling of nucleotides with aldehyde groups, followed by the exposure to Tollens reagent and a development solution, whereas the second method relies on the photoinduced deposition of Ag onto unmodified DNA samples. Several preparation parameters (DNA sequence, buffer salt type, Ag concentration, UV illumination time) are varied systematically.
The obtained DNA-templated nanoparticles are characterized with respect to their optical and structural properties. Plasmonic modes can be identified from maxima observed in the extinction spectra. The size and shape of the nanoparticles is inferred from conducting polarized dynamic-light-scattering experiments in the solution phase, as well as from imaging dispersed nanoparticles on singlecrystalline substrates by atomic force microscopy.
Language
English
HSG Classification
contribution to scientific community
Refereed
Yes
Publisher
N/A
Start page
1
Event Title
Molecular Plasmonics 2007
Event Location
Jena
Event Date
10.-12.05.2007
Subject(s)
Division(s)
Eprints ID
219265